Cai Zi-you
2011
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0
Influential Citations
1
Citations
Journal
Chinese Journal of Pharmaceutical Analysis
Abstract
Objective:To establish a new method for the content determination of moroxydine hydrochloride in moroxydine hydrochloride tablets by microfluidic chip with contactless conductivity detection.Methods:Moroxydine hydrochloride was rapidly separated by microfluidic chip and detected with contactless conductivity detection under high electric field.The electrophoretic parameters,such as the variety and concentration of buffer solution and additive,separation voltage and injection time,etc,were researched.Results:In optimal conditions,using 2 mmol·L-1 HAc+2 mmol·L-1 NaAc(pH=4.5) as buffer solution,with 0.3 mmol·L-1 SDS as additive at the separation voltage of 2.20 kV and 10 s injection,moroxydine hydrochloride was separated and detected within 2 min.The linear dependence of the concentration of moroxydine hydrochloride ranged from 20 μg·mL-1 to 200 μg·mL-1(r=0.9999),with the detection limit of 2.0 μg·mL-1(S/N=3),the RSD of 1.4% and the recoveries from 98.3% to 101.2%.Conclusion:The method is simple,rapid and reproducible,and it can be used for rapid detection and the quality control of the product.