A. Dankwardt, B. Hock, R. Simon
Nov 27, 1996
Citations
0
Influential Citations
24
Citations
Journal
Environmental Science & Technology
Abstract
2-Chloro-4-arylamino-6-alkyl-1,3,5-triazines were used as model compounds for non-extractable (bound) triazine residues. They were tested as targets in a competitive enzyme immunoassay with polyclonal and monoclonal antibodies originally raised against atrazine. Cross-reactivities were determined as a measure for the affinity of antibodies toward their respective analytes. 2-Chloro-6-isopropyl deriva tives were recognized as well as free atrazine (70−148% cross-reactivity, atrazine = 100%) with the exception of 2-chloro-4-[2‘-carboxy-4‘,5‘-dimethoxyanilino]-6-isopropylamino-s-triazine, carrying a carboxyl group in the arylamino side chain (<0.1−9% cross-reactivity). Functional group variation of positions 2 and 6 of the triazine ring resulted in cross-reactivities less than 15%. Arylamino-s-triazine samples from photolytic degradation experiments were analyzed by immunoassay and HPLC. Similar concentrations were obtained by both methods. Then the enzyme immunoassay was applied for the screening of triazin...