Yang Yan-hong
2010
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Journal
China Pharmacy
Abstract
OBJECTIVE:To determine the contents of ophiopogon Ra and cixi-ophiopogon A from Ophiopogon japonicus by HPLC.METHODS:The determination was performed on Waters C18(150 mm×4.6 mm,5μm) column with mobile phase consisted of acetonitrile-water(35:65) at flow rate of 1.0 mL·min-1.The detection wavelength was set at 208 nm and the column temperature was room temperature.RESULTS:The linear range of ophiopogon Ra was 1.88~9.40 μg(r=0.999 6) with an average recovery of 100.2%(RSD=1.7%,n=6).The liner range of cixi-ophiopogon A was 1.96~9.80 μg(r=0.999 5) with an average recovery of 99.7%(RSD=1.7%,n=6).CONCLUSION:The method is simple,rapid and accurate for the quality control of O.japonicus.