H. Nakazawa, Y. Kanamaru, A. Murano
Jul 25, 1979
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Journal
Chemical & Pharmaceutical Bulletin
Abstract
A method for separation and determination of optical isomers of 2, 2´-(4, 4´-cyclohexylidenediphenoxy)-2, 2´-dimethyldibutyric acid (clinofibrate) by high-performance liquid chromatography was developed. meso, d- and l-isomers of clinofibrate were derivatized with D-(+)-α-methylbenzylamine to the corresponding diastereomer amides, which were separated from one another by high-performance liquid chromatography. Each isomer in clinofibrate was determined. The chromatographic conditions were as follows : column, stainless-steel two connecting columns (150 mm in length and 4 mm in i.d.) packed with LiChrosorb SI-60 (5 μm) ; mobile phase, n-hexane/isopropanol (500/3, v/v) ; flow rate, 1.6 ml/min ; detector, UV photometer at 254 nm.