Wu Bin-bin
2013
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Journal
Chinese Journal of Experimental Traditional Medical Formulae
Abstract
Objective:To determine the content of piefeltarraenin IA in different medicinal parts of Picria fel-tarrae in different growth periods.Method: High performance of liquid chromatography(HPLC) was adopted,the separation was performed on C18 column with acetonitrile-0.1% aqueous acetic acid solution(36∶64) as mobile phase,the flow rate was 1 mL·min-1,the detection wavelength was set at 264 nm and the column temperature was kept at 25 ℃.Result: From the last year in June to the next year in January,average content of piefeltarraenin IA in leaves were listed monthly as follows: 0.37%,0.66%,0.59%,0.45%,0.35%,0.34%,0.31%,0.23%;and in the stems: 0.24%,0.38%,0.36%,0.24%,0.13%,0.08%,0.03%,0.02%;the content of piefeltarraenin IA in roots was 0.18% in July and was not detected in other times of the whole year.Conclusion: The content of piefeltarraenin IA was highest in July and August,regarded the content of piefeltarraenin IA as standard,the optimum collection time of Picria fel-tarrae was in July and August.This research could provide scientific basis for optimizing the collection time and actualizing standard planting of Picria fel-tarrae.