T. Wu, C. Wang, X. Wang
Oct 1, 2008
Citations
1
Influential Citations
44
Citations
Journal
International Journal of Cosmetic Science
Abstract
An ultra performance liquid chromatography (UPLC) method has been developed for the simultaneous determination of 21 preservatives: 2‐methyl‐4‐isothiazoline‐3‐ketone, bronopol, 5‐chloro‐2‐methyl‐4‐isothiazoline‐3‐ketone, benzyl alcohol, 2‐phenoxyethanol, methyl‐p‐hydroxy benzoate, ethyl‐p‐hydroxy benzoate, methyl benzoate, 4‐hydroxybenzoic acid iso‐propyl ester, propyl‐p‐hydroxy benzoate, 4‐chloro‐3‐methylphenol, ethyl benzoate, 2‐phenylphenol, 4‐hydroxybenzoic acid iso‐butyl ester, butyl‐p‐hydroxy benzoate, 4‐chloro‐3,5‐dimethylphenol, phenyl benzoate, 2,4‐dichloro‐3,5‐dimethylphenol, 2‐benzyl‐4‐chlorophenol, triclocarban and triclosan in cosmetics. A Waters ACQUITY UPLC BEH C18 column was used with 0.1% formic acid solution as the mobile phase under the condition of gradient elution. Preservatives were extracted with methanol by ultrasonicator, and then they were analysed by UPLC‐PDA detector. All these preservatives were baseline separated in 8.5 min. The pre‐treatment method of samples and the chromatographic condition of analysis were critically examined in this study. The recoveries ranged from 90.5 to 97.8%, with RSD values below 3.2%, and all correlation coefficients (r) were no less than 0.9997. Thus, this method could be used for analysing the preservatives in cosmetic products.