G. Santoni, P. Mura, S. Pinzauti
Oct 30, 1990
Citations
0
Influential Citations
11
Citations
Journal
International Journal of Pharmaceutics
Abstract
Abstract A UV spectroscopic method for the simultaneous determination of procaine hydrochloride and phenazone in eardrops was developed. The individual concentrations were calculated by means of a previously described algorithm (using factors based on absorbance ratios) bundled to the computer-aided apparatus. The selected wavelengths were 290 and 242 nm, corresponding to the λ max of procaine hydrochloride and phenazone, respectively. The method allows rapid and accurate determination of the binary mixture m the tested concentration range of 2–9 μg ml −1 for procaine hydrochloride and 10–40 μg ml −1 for phenazone, with a relative standard deviation of less than 1.8%. Second-order derivative spectroscopy and zero-crossing derivative methodology were tested but were found to be unsuitable in this case.