Cao Yun-fei
2007
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Journal
Qilu Pharmaceutical Affairs
Abstract
OBJECTIVE To determine the content of protocatechualdehyde in Ruan Gan Granules by RP-HPLC.METHODS The determination was carried out with Symmetry C18column.The mobile phase consisted of CH3OH-1% acetic acid(12∶88).The flow rate was 1mL·min-1 and the detective wavelength was 279nm.RESULTS The content of protocatechualdehyde can be determined by RP-HPLC.The linear concentration ranges of these compound were 0.0552~0.2208μg.The average recoveries were 99.9%,RSD was 0.5%.CONCLUSION The method was simple and accurate and can be used to control the quality of Ruan Gan Granules.