G. Vampa, S. Benvenuti, P. Pecorari
Jul 3, 1992
Citations
0
Influential Citations
2
Citations
Journal
Journal of Chromatography A
Abstract
Abstract A high-performance thin-layer chromatographic method is described for the investigation of the methylation rates of uracil, 5-fluoro-, 5-chloro-, 5-bromo-, 5-methyl- and 5-nitrouracil and their N1- and N3-methyl derivatives. The method allowed the amounts of compounds obtained, the time required to complete the reaction and therefore the reactivity of the uracil derivatives to be determined. The N1- and N3-methyl and N1,3-dimethyl derivatives were synthesized and separated by droplet countercurrent chromatography and/or medium-pressure liquid chromatography and their RF values were determined in different solvent systems by thin-layer chromatography.