Qin Shude
2011
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Journal
Northwest Pharmaceutical Journal
Abstract
Objective To develop an HPLC method for simultaneous determination of deacetylasperulosidic acid and scandoside in Hedyotis diffusa.Methods The separation was performed on a Pinnacleu C18 column(250 mm×4.6 mm,5 μm) with methanol-acetonitrile-water(3∶2∶95,containing 1 mol·L-1 acetic acid) as the mobile phase at the flow rate of 1.0 mL·min-1.The UV detection was set at 230 nm.Results The calibration curve of deacetylasperulosidic acid and scandoside were linear in the concentration range of 12-240 μg·mL-1(r=0.999 7) and 10.8-216 μg·mL-1(r=0.999 8).The average recoveries of deacetylasperulosidic acid and scandoside were 101.1% and 99.8%,respectively.The RSD of deacetylasperulosidic acid and scandoside were 2.8% and 2.3%,respectively.Conclusion The method is simple,accurate and reproducible.It can be used for determining deacetylasperulosidic acid and scandoside simultaneously in Hedyotis diffusa.