D. L. Simmons, R. Ranz, P. Picotte
Sep 20, 1972
Citations
0
Influential Citations
18
Citations
Journal
Journal of chromatography
Abstract
Abstract A gas chromatographic method utilizing a flame ionization detector is described for the determination of tolbutamide in serum. The drug is extracted from acidified serum (pH 5.4), methylated with dimethyl sulfate and the resulting N-methyltolbutamide is determined by gas chromatography against an internal reference standard, N-propyl-p-chlorobenzenesulfonamide. Under suitable conditions, N-methyltolbutamide is quantitatively pyrolyzed to N-methyl-p-toluenesulfonamide. Peak areas for equimolar concentrations of these two compounds were similar and linear with respect to the internal standard over a range of 10 to 50 μg of tolbutmide equivalents per 100 μl. Overall recoveries from human and beagle serum were 80.8 ± 3.5% and 82.0 ± 3%, respectively, based on the N-methyltolbutamide external standard curve.