Suzuki Masaaki, Ueno Takashi, Taka Tetsuo
Sep 10, 1981
Citations
0
Influential Citations
4
Citations
Journal
Clinica Chimica Acta
Abstract
Abstract A rapid and sensitive fluorometric rate assay of serum cystine aminopeptidase is described using a novel fluorogenic substrate, 7- S -benzyl- l -cysteinyl-4-methylcoumarinylamide. The proposed method enabled us to determine cystine aminopeptidase activity in one minute using 10 μl of serum. The assay was shown to be free from error due to the adsorption of the substrate to serum albumin. The values obtained by this method gave good correlation with those obtained by the conventional method of Watson [6].