D. Grassetti, J. B. Murray
1967
Citations
7
Influential Citations
793
Citations
Quality indicators
Journal
Archives of biochemistry and biophysics
Abstract
Abstract A procedure is described that permits determination of SH groups in simple compounds and in biological materials. The procedure utilizes the reaction of 2,2′-dithiodipyridine or of 4,4′-dithiodipyridine with thiols, which gives the corresponding 2- or 4-thiopyridone. The ultraviolet absorption of the thiopyridone formed is measured. The method permits determination of less than 1.5 μg SH when 2,2′-dithiodipyridine is used, and of less than 0.5 μg SH when 4,4′-dithiodipyridine is used. The proposed method is compared with known methods for the determination of sulfhydryl groups.