Hu Jian-lin
2010
Citations
0
Influential Citations
0
Citations
Journal
Journal of Kunming Medical University
Abstract
Objective To establish a method to determine the content of Wilforlide A in Tripterygium hypoglaucum(Levl.)Hutch by HPLC.Methods The content of Wilforlide A was measured by HPLC on reversed-phase C18 column with acetonitrile-water(80:20)as mobile phase and 205 nm as the detection wavelength.Result The content of Wilforlide A was linear in the range of 0.42~10.5 μg/mL with a regression coefficient of 0.995,the average rate of recovery was 94.3%,RSD was 2.87%.Conclusion The method is sensitive and accurate with good reproducibility.