H. Zhang
2014
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0
Influential Citations
2
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Journal
China Pharmacy
Abstract
OBJECTIVE:To establish the method for the content determination of wilforlide in Tripterygium wilfordii from different producing area. METHODS:HPLC method was adopted. The determination was performed on Welch material Inc C18(250mm×4.6 mm,5 μm)column with mobile phase consisted of methanol-0.1% phosphate(containing 0.2% triethylamine,85 ∶ 15,V/V)at the flow rate of 1.0 ml/min. The detection wavelength was set at 210 nm,and the column temperature was 25 ℃. RESULTS:The linear range of wilforlide was 0.20-2.00 μg(r=0.999 9)with an average recovery of 97.86%(RSD=1.34%,n=6).RSDs of precision,stability and reproducibility tests were lower than 1%. CONCLUSIONS:The method is simple,accurate,reproducible and suitable for the content determination and quality control of wilforlide in T. wilfordii and related preparations.