Li Hong-lin
2015
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Journal
Chinese Journal of Pharmaceutical Analysis
Abstract
Objective: To establish an HPLC method for the determination of five kinds of nucleotides [including cytidine 5’-monophosphate disodium salt( 5’-CMPNa2),uridylic-5’-monophosphate disodium salt( 5’-UMPNa2),guanosine-5’-monophosphate disodium salt( 5’-GMPNa2),inosine 5’-monophosphate disodium salt( 5’-IMPNa2),5’-adenylic acid]in nucleotide pollen capsules. Methods: The chromatographic separation was achieved on a Waters Atlantis T3 column( 4. 6 mm × 250 mm,5 μm),using 10 mmol·L -1 KH2PO4 buffer solution( adjusted to p H 3. 9 with phosphoric acid) as the mobile phase at a flow rate of 1. 0 m L·min -1 ,and the detection wavelength was set at 260 nm. Results: Five nucleotides were well separated within 25 minutes. The five calibration curves of nucleotides showed good linearity with correlation coefficient of 0. 999 4-0. 999 7. The average recovery ranges( n =6) were between 88. 25% and 101. 3% with RSDs of 0. 94%-4. 04%. In the 3 batches of samples,the total contents of nucleotides were 293 mg·g -1 ,287 mg·g -1 and 289 mg·g -1 . Conclusion: The method is simple,rapid,sensitive,reproducible,accurate and reliable,which can meet the needs of daily analytical testing,and can be applied to the quality control of five nucleotides in health products.