M. Eisenberg, K. Krell
Oct 25, 1969
Citations
1
Influential Citations
19
Citations
Journal
The Journal of biological chemistry
Abstract
Abstract Dethiobiotin synthesis from 7,8-diaminopelargonic acid was demonstrated in cell-free extracts of Escherichia coli bioA mutants. The biosynthetically formed dethiobiotin was identified by its avidin combinability, by various chromatographic techniques, and by its differential growth response for several biotin auxotrophs. Requirements for the reaction include bicarbonate, ATP, and Mg2+, as well as 7,8-diaminopelargonic acid. The ureido carbonyl group of dethiobiotin was exclusively labeled when the reaction was carried out in the presence of NaH14CO3. The enzyme was absent from a mutant deleted for the entire bioA operon and from mutants which map within one bioA cistron. The dethiobiotin and 7-keto-8-aminopelargonic acid synthetase enzymes were found to be coordinately repressed by biotin.