Ji-eun Kwon, Heejung Kim, J. Do
Sep 30, 2014
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Abstract
Fluxapyroxad is classified as carboxamide fungicide that inhibits succinate dehydrogenase in com- plex II of mitochondrial respiratory chain, which results in inhibition of mycelial growth within the fungus target spe- cies. This study was carried out to assure the safety of fluxapyroxad residues in agricultural products by developing an official analytical method. A new, reliable analytical method was developed and validated using High Performance liquid Chromatograph- UV/visible detector (HPLC-UVD) for the determination of fluxapyroxad residues. The flux- apyroxad residues in samples were extracted with acetonitrile, partitioned with dichloromethane, and then purified with silica solid phase extraction (SPE) cartridge. Correlation coefficient(R 2 ) of fluxapyroxad standard solution was 0.9999. The method was validated using apple, pear, peanut, pepper, hulled rice, potato, and soybean spiked with flux- apyroxad at 0.05 and 0.5 mg/kg. Average recoveries were 80.6~114.0% with relative standard deviation less than 10%, and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 and 0.05 mg/kg, respectively. All val- idation parameters were followed with Codex guideline (CAC/GL 40). LC-MS (Liquid Chromatograph-Mass Spec- trometer) was also applied to confirm the analytical method. Base on these results, this method was found to be appropriate fluxapyroxad residue determination and can be used as the official method of analysis.