V. Ravikumar, M. Andrade, Recaldo L. Carty
May 1, 2006
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0
Influential Citations
13
Citations
Journal
Bioorganic & medicinal chemistry letters
Abstract
Efficient synthesis of phosphorothioate RNA (PS-RNA) is demonstrated by using phenylacetyl disulfide (PADS) in a mixture of pyridine and acetonitrile (1:1, v/v) for 3 min. Sulfurization is achieved with >99.8% stepwise efficiency. This reagent also performs efficiently during synthesis of RNA containing PS:PO mixed backbone.