R.V. Bharucha, Usha Parmar
Aug 15, 2013
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Abstract
Meropenem and Sulbactam sodium as combination injection is used for the treatment of lower respiratory tract infection caused by gram negative bacteria in adults only. The aim of this research work to developed and validate simple, specific and sensitive RP-HPLC method for estimation of Meropenem and Sulbactam sodium in combined pharmaceutical dosage form. In RP-HPLC, method was carried out by isocratic technique on a reversed-phase. HyperSil C18 column kept at ambient temperature and UV detection at 225 nm with mobile phase containing a mixture of water with 0.2% triethylamine and Acetonitrile pH 6.0 adjusted with Ortho-phosphoric acid (80:20 v/v), at a flow rate of 1.0 ml/min. Calibration curves were linear in the concentration range of 8-18 μg/ml for MPM (r 2 = 0.9998) and SUL 4-9 μg/ml for SUL (r 2 = 0.9995).the method was validated in terms of accuracy, precision ,interday, intraday and robustness as per ICH guideline. Thus all three methods were found to be simple, sensitive, accurate, precise so these methods applicable for routine analysis.