M. Piponski, Irena Slaveska Spirevska, Tanja Bakovska Stoimenova
Dec 31, 2022
Citations
0
Influential Citations
0
Citations
Journal
Macedonian Pharmaceutical Bulletin
Abstract
Benzydamine hydrochloride is a non-steroidal antiinflammatory drug with local anesthetic and analgesic properties providing both rapid and extended pain relief, as well as a significant anti-inflammatory treatment for the painful inflammatory conditions of the mouth and throat (Sugiarto et al., 2020). Cetylpyridinium chloride is a quaternary pyridinium antiseptic with bactericidal activity against Gram-positive and, at higher concentration, some Gram-negative bacteria. It is use as lozenges, gels or solutions for the treatment of minor infections of the mouth and throat (Steyer et al., 2021). There are a number of methods available for determination of Benzydamine hydrochloride and Cetylpyridinium chloride, individually (Ph.Eur., BP, USP), but only a few methods for simultaneous determination of a combination of them in final dosage forms. The aim of this study was to develop simple, fast and efficient reverse phase high performance liquid chromatography (RP-HPLC) method for simultaneous determination of Benzydamine hydrochloride and Cetylpyridinium chloride in final dosage form preparations and validate this method in accordance with current International Conference on Harmonization (ICH) Analytical Method Validation requirements. Materials and methods