P. Yang, Haibin Hu, Yang Liu
Feb 3, 2018
Citations
2
Influential Citations
61
Citations
Journal
Aquaculture
Abstract
Abstract Recent studies have revealed the beneficial effects of stachyose on intestinal histology and digestive function of fish. However, a comprehensive understanding of stachyose's impact on intestinal health of fish remains unclear, limiting its use in aqua-feed. In the present study, a 12-week feeding trial was conducted to investigate the effects of dietary stachyose on intestinal microbiota and mucosal barrier function of turbot (S. maximus L). Three isonitrogenous and isolipidic experimental diets were formulated to contain 0%, 1.25% and 5% stachyose, respectively. Sequencing of bacterial 16s rRNA V4 region indicated that dietary stachyose altered the intestinal adherent microbiota profile, which was supported by the diet-cluster of PCA, PCoA, beta diversity heatmap and phylogenetic tree. LEfSe and MetaStat analysis indicated that both 1.25% and 5% dietary stachyose significantly elevated the abundance of intestinal cellulose-degrading bacteria. However, the higher level of stachyose (5%) increased the abundance of intestinal beneficial bacteria as well as that of potential pathogenic bacteria. Moreover, 1.25% dietary stachyose significantly up-regulated the genes expression of occludin, claudin-3, and ZO-1, and down-regulated the gene expression of claudin-like in the intestine (P