J. Gayarre, K. Stamatakis, M. Renedo
Oct 24, 2005
Citations
0
Influential Citations
52
Citations
Journal
FEBS Letters
Abstract
Cyclopentenone prostaglandins (cyPG) with antiinflammatory and antiproliferative properties have been envisaged as leads for the development of therapeutic agents. Because cyPG effects are mediated in part by the formation of covalent adducts with critical signaling proteins, it is important to assess the specificity of this interaction. By using biotinylated derivatives of 15‐deoxy‐Δ12,14‐PGJ2 (15d‐PGJ2‐B) and PGA1 (PGA1‐B) we herein provide novel evidence for the differential selectivity of protein modification by distinct cyPG. The marked quantitative and qualitative differences in the binding of 15d‐PGJ2‐B and PGA1‐B to cellular proteins were related to a differential reactivity in the presence of glutathione (GSH), both in vitro and in intact cells. Therefore GSH levels may influence not only the intensity but also the specificity of cyPG action.