Ziwen Liu, Yumei Fan, Yu Wang
May 28, 2008
Citations
1
Influential Citations
35
Citations
Quality indicators
Journal
FEBS Letters
Abstract
Dipyrithione (PTS2) possesses anti‐bacterial and anti‐fungal activity. In the present study, we found that PTS2 dose‐dependently inhibited the LPS‐induced up‐regulation of nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2) protein level in RAW264.7 cells. RT‐PCR experiments showed that PTS2 suppressed LPS‐induced iNOS but not COX‐2 expression at the mRNA level. As expected, PTS2 prevented NO secretion in RAW264.7 cells. Furthermore, PTS2 administration significantly decreased LPS‐induced mortality in mice. Mechanistically, PTS2 decreased expression and phosphorylation of STAT1, but did not interfere with the MAPK and NF‐κB pathways. In conclusion, PTS2 protects mice against endotoxic shock and inhibits LPS‐induced production of pro‐inflammatory mediators, suggesting that PTS2 could play an anti‐inflammatory role in response to LPS.