S. Woolfrey, G. Taylor, I. Kellaway
Sep 1, 1985
Citations
0
Influential Citations
10
Citations
Quality indicators
Journal
International Journal of Pharmaceutics
Abstract
Abstract The pharmacokinetics of 6-carboxyfluorescein (CF) have been studied in the rat. CF was rapidly removed from blood in a biphasic manner and was found to have a small volume of distribution together with a clearance approaching renal blood flow. Purification of commercial CF resulted in a significantly larger volume of distribution, but insignificant changes in clearance and half-lives, as estimated using data from the commonly employed fluorimetric assay. Purified CF pharmacokinetics were linear following intravenous doses of 0.5–2 mg · kg −1 ; however, at 4 mg · kg −1 both clearance and volume of distribution were found to be dose-dependent. In rats anaesthetized with urethane, CF clearance was 37% lower than in rats anaesthetized with pentobarbitone. This difference in clearance was reflected by a large increase in elimination half-life during urethane anaesthesia.