P. W. Aschbacher, C. Struble, V. Feil
Nov 1, 1995
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Quality indicators
Journal
Journal of Agricultural and Food Chemistry
Abstract
Disposition of oral sulfathiazole was studied in swine. Pigs were slaughtered 6, 12, 24, and 48 h after an oral dose of [ 14 C]sulfathiazole (two at each time period). Excretion of 14 C was rapid (>90% in 48 h), primarily via the urine. Metabolites isolated and characterized by 1 H NMR and FAB MS were N 4 -acetylsulfathiazole from urine, kidney, liver, blood, and muscle ; N 4 -glucoside of sulfathiazole from muscle ; and an apparent diconjugate from liver, a glucuronide of N4-acetylsulfathiazole. Quantitation was accomplished by HPLC analysis of samples (extracts of tissue and urine) spiked with the reference compounds. Peaks corresponding to the retention time of the reference compounds were trapped and assayed for 14 C. Sulfathiazole and N4-acetylsulfathiazole were the principal 14 C-labeled compounds in urine and kidney. If present, the glucoside or glucuronide represented <5% of the 14 C in urine. Liver and muscle contained significant amounts of sulfathiazole, N 4 -acetylsulfathiazole, and N 4 -glucoside of sulfathiazole. Quantitation of the diconjugate was not attempted. The amounts isolated suggest it was a minor metabolite ; however, instability during isolation was apparent. On the basis of the results, liver, kidney, and urine are potential target tissues for a residue monitoring program and the nature of the metabolite present in the tissue must be considered when an assay procedure is selected.