R. Bradford, R. Shapira, D. Doherty
Nov 1, 1961
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Journal
International journal of radiation biology and related studies in physics, chemistry, and medicine
Abstract
SummaryBoth the tissue distribution and intracellular distribution of mercaptoethylguanidine hydrobromide (MEG), d-mercaptobutyl-2-guanidine hydrobromide (d-MBG-2), and l-mercaptobutyl-2-guanidine hydrobromide (L-MBG-2) were studied in an attempt to determine how these radiation-protective compounds may function in vivo. The tissue distributions of all three compounds, which differ by as much as 5 : 1 in their protective activity in mice, were similar, both qualitatively and quantitatively. Their intracellular distribution in mouse liver, rat liver, and rat spleen was selective. Furthermore, a relation between protective activity and intracellular distribution was observed. The intracellular-distribution pattern was altered when the compound was given after irradiation. It is inferred that the microsomal fraction may be involved in radiation death.An investigation of the nature of the binding of each of these compounds to the cellular constituents was made. Dialysis techniques were used in several solvent...