James Hill, David E. Griffiths
Oct 25, 1991
Citations
19
Influential Citations
532
Citations
Quality indicators
Journal
Nucleic acids research
Abstract
The transformation efficiency of Saccharomyces cerevisiae by the lithium acetate (LiOAc) method (1) is low compared with spheroplast (2) and electroporation (3) protocols. It is, however, quick and simple, making it ideal when a high efficiency of transformation is not essential. Dimethyl sulfoxide (DMSO) has previously been shown to enhance transformation of prokaryotes (4) and eukaryotes (5). We report a DMSO-modified version of