Kohei Yamamoto, A. Shibahara, T. Nakayama
Nov 1, 1991
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0
Influential Citations
8
Citations
Journal
Lipids
Abstract
Double-bond locations in heneicosapentaenoic acid from eel lipids were determined by a method involving gas chromatography/mass spectrometry (GC/MS). The methyl esters of the pentaenoate fraction were first partially reduced with hydrazine. The resultingcis-monoenoates were then methylthiolated, and the resultant adducts were analyzed by GC/MS. The key fragmentation ions generated by the cleavage between the methylthio-substituted carbons were used to ascertain the original double-bond positions in the native fatty acid esters. Based on mass spectral evidence, the acid was identified as all-cis-6,9,12,15,18-heneicosapentaenoic acid.