A. Russfield, Freddy Homburger, Eliahu Boger
1975
Citations
1
Influential Citations
77
Citations
Quality indicators
Journal
Toxicology and applied pharmacology
Abstract
Abstract As part of a series of bioassays of industrial and environmental chemicals for chronic toxicity or carcinogenicity, 4,4′-methylene-bis-(2-chloroaniline) was tested in male and female randombred albino mice and male Charles River CD rats. Preliminary studies established that the maximally tolerated dose of the chemical in the diet was 1000 mg/kg in rats and 2000 mg/kg in mice. The compound, at these dose levels and half the level, was fed to male and female mice, and male rats for 18 months, followed by control diet for another 6 months. In female mice but not in males, a statistically significant incidence of hepatoma at both dose levels was observed. In addition, a higher incidence of tumors was observed in treated animals than in controls as follows: hepatomas and lung adenomatosis in rats, hemangiosarcomas and hemangiomas in mice. Thus, 4,4′-methylene-bis-(2-chloroaniline) is definitely carcinogenic in female mice.