V. Dharuman, J. Hahn
Nov 15, 2007
Citations
1
Influential Citations
36
Citations
Journal
Sensors and Actuators B-chemical
Abstract
Abstract Influence of short chain alkane diluents (number of carbons, n = 2, 3 and 6) on the control of non-specific DNA-gold (DNA-Au) interactions and hybridization discrimination effect has been investigated at the binary monolayer interface consisting of 6-mercapto-1-hexane labeled single stranded DNA (HS-ssDNA) and a diluent (HS-ssDNA/diluent) in presence of double stranded DNA (dsDNA) specific cationic intercalators and [Fe(CN)6]3−. The diluents used are 1-mercaptopropane (MP), 3-mercaptopropionic acid (MPA), Cystamine, 1-mercapto ethanol (MCE) and 6-mercapto-1-hexanol (MCH). Label free electrochemical DNA hybridization discrimination of the complementary hybrid (hybridized) and non-complementary (un-hybridized) surfaces has been made comparatively in presence of the [Fe(CN)6]3− and dsDNA specific intercalators, such as proflavine and methylene blue. Prior to the target hybridization, the control of non-specific DNA-Au interaction is interrogated by monitoring the integrity of the HS-ssDNA monolayer in presence of the [Fe(CN)6]3− before and after the HS-ssDNA/diluent binary interface formation. Change in the peak-to-peak separation (ΔEp), taken as a measure of the control of the non-specific DNA-Au interactions, follows the order MP