S. Skupp, G. Vugrek, J. Ayvazian
Nov 15, 1979
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0
Influential Citations
11
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Quality indicators
Journal
Biochemical pharmacology
Abstract
Abstract The effect of erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) on purine, pyrimidine and ribonucleic acid metabolism in the human peripheral lymphocyte during the early phases of blastogenesis has been studied. Experiments that determine the incorporation of radioactive free base or nucleoside into their respective nucleotides indicated EHNA-inhibited phosphoribosylpyrophosphate-dependent reactions, nucleoside kinase reactions and RNA synthesis. Studies utilizing the separating and quantitating ability of high pressure liquid chromatography demonstrated that 5–10 μM EHNA increased the nucleotide pool size of ADP without significantly changing the pool sizes of ATP, GTP or UTP. It also greatly increased the incorporation of deoxy-adenosine into dADP and dATP. Higher concentrations of EHNA (15–100 μM) reduced all the measured nucleotide pool sizes when compared to the values observed at the lower EHNA concentration. In addition, 5–10 μM EHNA significantly reduced the energy level of the cell, which was not further altered by higher concentrations of EHNA. Our studies also determined that EHNA inhibited human lymphocyte guanase and indirectly increased glycolysis and respiration.