C. Greenspan, I. Wilson
Sep 1, 1970
Citations
0
Influential Citations
4
Citations
Quality indicators
Journal
Molecular pharmacology
Abstract
Methanesulfonates [(3-hydroxyphenyl)trimethylammonium iodide methanesulfonate, 1, 19-pentamethylenebis(3-hydroxypyridinium iodide) methanesulfonate, and methanesulfonyl fluoride] react with acetylcholinesterase to form an inactive methaneusulfonyl-enzyme derivative analogous to the acetyl-enzyme that is formed during the hydrolysis of esters. The methanesulfonyl-enzyme is desulfonylated by reaction with thiocholine. When the effect of fluoride on these two steps was studied, it was found that fluoride inhibits sulfonylation but does not affect desulfonylation. It was also found that fluoride and a quaternary ammonium ion can bind simultaneously to the enzyme. In one case the binding was independent, in many cases it was partially competitive, in one case it was completely competitive, and in two cases it was cooperative.