Xiao-hua Guo
2012
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Quality indicators
Journal
Chinese Journal of Arteriosclerosis
Abstract
Aim To observe the effect of ibrolipim on the expresstion of endothelin-1(ET-1) and von Willebrand factor(vWF) in cultured human umbilical vein endothelial cells induced by high glucose,and to investigate its molecular mechanism.Methods Human umbilical vein endothelial cell-12(HUVEC-12) were cultured respectively under normal glucose level(5.5 mmol/L),high glucose level(33.3 mmol/L),and high glucose level with ibrolipim treatment.HUVEC-12 activity or injury was assayed by methyl thiazolyl tetrazolium(MTT).The ET-1 concentrations were evaluated by radioimmunoassay.The expression of vWF in the conditioned media was tested by enzyme-linked immunosorbent assay(ELISA).Endothelial dysfunction was identified by the expression of ET-1 and vWF through reverse transcription polymerase chain reaction(RT-PCR).The protein content of ET-1 and vWF were evaluated by cell immunofluorescence.Results High glucose condition significantly weakened cell viability,and increased the expression of ET-1 and vWF.Ibrolipim treatment significantly attenuated these alterations of endothelial dysfunction.The low er con-cen trations(2,4,8 μmol/L) of ibrolipim improved cell viability,down-regulated the expression of ET-1 and vWF,and attenuated the cytotoxicity,however,higher concentration(16,32 μmol/L) of ibrolipim aggravated the damage of HUVEC-12 cultured under high glucose level.Conclusion Ibrolipim at low concentration can inhibit high glucose-induced endothelial dysfunction of cultured HUVEC-12,which may be related to the alternation of ET-1 and vWF expression.