W. Jian
2007
Citations
0
Influential Citations
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Citations
Quality indicators
Journal
Chinese Journal of Gerontology
Abstract
Objective To explore the inhibitive effect of the self-made cysteinyl-arginyl-glycyl-aspartyl-tryptophanyl-penicillamine (Cys-Arg-Gly-Asp-Trp-Pen,W2003,i.e.Arg-Gly-Asp peptides platelet suppressant drug) on haman platelet aggregation and thrombogene- sis in the rats and compare the effect of N~α-acetyl-arginyl-glycyl-aspartyl-phenethylamine (AcArg-Gly-Asp-NHCH_2CH_2C_6H_5,W2001) and Nα-Ac-Arg-Gly-Asp-p-methoxyl phenylethylamine (W2002) reported before.Methods Platelet aggregation induced by adenosine diphos- phate (ADP) was detected by turbidity method and platelet count.W2003 competitive inhibition on binding of ~(125)Ⅰfibrinogen (FGN) and platelet glycoprotein (GP)Ⅱb/Ⅲa were measured by radioligand assay.Arterial thrombus rats model were made by 20% FeCl_3 stimulation on common carotid artery of Wistar rats to evaluate the preventive effect of this compound on thrombogenesis by thrombus wet and dry weight of the stimulated artery section.Results W2003 significantly inhibited platelet aggregation induced by ADP,there was significant difference between different dosages (150,100,50,10 and 1μmol/L) and saline (P0.01).IC50 value of inhibiting binding of ~(125)ⅠFGN and GPⅡb/Ⅲa was (31.5±7.0)μmol/L.The order of preventing thrombogenesis from strong to feeble was W2003W2001W2002,differing from saline (P0.01).Conclusions W2003 could play the inhibitive role in platelet aggregation and thrombegenesis through suppressing the binding of ~(125)ⅠFGN and GPⅡb/Ⅲa.