R. Asaoka, Tetsushi Nakagamil, Haogang Zhu
Jun 1, 2009
Citations
0
Influential Citations
3
Citations
Quality indicators
Journal
Cutaneous and Ocular Toxicology
Abstract
Purpose: To investigate the effects of levocabastine hydrochloride on the profileration, Deoxyribonucleic acid (DNA) synthesis and induction of apoptosis, in human Tenon’s capsule fibroblasts. Methods: The maximum intoxicate concentration was determined by measuring the release of lactate dehydrogenase. Proliferation of fibroblasts was analyzed with a WST-1 method. DNA synthesis was examined by quantifying the incorporation of 5-bromo-2’-deoxyuridine into dividing cells, and apoptosis was analyzed using an Annexin-V-FLUOS Staining Kit. Results: Levocabastine hydrochloride (10−4 M), ketotifen fumarate (3 × 10−5 M) and tranilast (10−4 M) significantly inhibited proliferation of fibroblasts. Levocabastine hydrochloride (10−4 M), ketotifen fumarate (10−4 M) and tranilast (10−3 M) inhibited DNA synthesis and induced apoptosis. Conclusion: Levocabastine hydrochloride inhibited the proliferation of fibroblasts.