H. Don
2014
Citations
0
Influential Citations
0
Citations
Quality indicators
Journal
Chinese Journal of Experimental Traditional Medical Formulae
Abstract
Objective: To investigate effect of licoflavone on blood glucose,glycogen,muscle glycogen expression of retinol-binding protein 4( RBP4) of adipose tissues in rats after exhaustive exercise. Method: Fifty healty male Sprague-Dawley rats were randomized into three groups,10 rats in each group; the quiet control group( NC),the exercise group( ME),the low. middle and high dose licoflavone combined with exercising group ( MGFL,MGFM,MGFH,4,8,12 g·kg- 1·d- 1). The rats were administrated by gavage once a day half an hour before exercising for 6 days in one week, and lasted for six weeks. The control group was administrated physiological saline. The rats were killed after 6-weeks. The contents of serum glucose,glycogen,muscle glycogen were tested by corresponding kit method. And the expression of epididymal adipose tissue RBP4 mRNA in rats was detected by reverse transcription-polymerase chain reaction( RT-PCR) method. Result: The exhaustive swimming training induced the contents of serum glucose,glycogen,muscle glycogen in the exercise group and each MGF group was significantly lower than in the quiet control group( P 0. 05 or P 0. 01). The content of serum glucose was respectively low( 4. 81 ± 0. 24),( 5. 31 ± 0. 12),( 5. 78 ± 0. 22),( 5. 92 ± 0. 18) mmol·L- 1. The content of serum glucose in each MGF group was higher than in the exercise group. And there was a significant difference between MGFH and the exercise group( P 0. 05). The content of muscle glycogen was respectively low( 0. 91 ± 0. 16),( 0. 98 ± 0. 14),( 1. 34 ± 0. 12),( 1. 46 ± 0. 23) mg·g- 1. The content of muscle glycogen in each MGF group was higher than the exercise group. And there were significant difference between MGFM,MGFH and the exercise group( P 0. 05). The content of glycogen was respectively decreased( 6. 21 ± 1. 10),( 8. 92 ± 1. 11),( 9. 18 ± 1. 13),( 10. 16 ± 1. 16) mg·g- 1. And the content of glycogen in each MGF group was higher than that in the exercise group( P 0. 05 or P 0. 01). The expression of epididymal adipose tissue RBP4 mRNA in exhaustive swimming group and each MGF group increased( 1. 430 ± 0. 123),( 1. 101 ± 0. 103),( 0. 962 ± 0. 112),( 0. 926 ± 0. 101). The expression of RBP4 mRNA in the exercise group had a significant difference compared to the quiet control group( P 0. 05). The expression of RBP4 mRNA in each MGF group was lower than that in the exercise group,and the expression of epididymal adipose tissue RBP4 mRNA in MGFM and MGFH groups had significant difference compared to the exercise group( P 0. 05). Conclusion: The supplement of licoflavone could maintain the increase of glycogen reserve,the relatively stable blood glucose concentration and down regulate the expression of RBP4 mRNA. This is contributed to improving the athletic ability of rats.