Zhao Xing-zeng
2010
Citations
0
Influential Citations
2
Citations
Quality indicators
Journal
Natural Product Research and Development
Abstract
The cytotoxic effects and the molecular mechanism of Macranthoside B(MB) were investigated in this study.The cytotoxic effects of MB for LOVO and HL-60 cells were detected by MTT assay.Using RT2 ProfilerTM PCR Array,84 cancer-related genes were evaluated by a high-throughput quantitative real-time RT-PCR with or without MB treatment.Cell viability assay showed that MB inhibited cell growth of two cancer cell lines,especially in human acute promyelocytic leukemia HL-60 cells.As shown in microarray,a total of 20 differential genes were found,14 of which were upregulated and 6 were down-regulated.Their biologic functions demonstrate that MB may exhibit anti-tumor activity against HL-60 cells by arresting cell cycle and reducing cell metastasis.