B. Fox, M. Fox
Nov 1, 1967
Citations
0
Influential Citations
9
Citations
Quality indicators
Journal
Cancer research
Abstract
The effect of methyl methanesulfonate, ethyl methanesulfonate, isopropyl methanesulfonate, and methylene dimethanesulfonate on the survival of the mouse lymphoma line, P388F, is described. The concentrations of alkanesulfonate resulting in a 50% inhibition of growth after a 3-hr exposure were 0.1 mm, > 16 mm, > 1.5 mm, and 0.037 mm, respectively. A more detailed examination of the action of methyl methane-sulfonate with regard to the time of onset of inhibition of nucleic acid and protein synthesis was made. The relative uptakes of thymidine-3H, uridine-5-3H, glycine-3H, and tyrosine-3H were investigated and compared. Methyl methanesulfonate affects differentially the ability of the cell membrane to transport different macromolecule precursors into the cell pool. The potential causes of this differential action are discussed in relation to the known sensitivyt ot alkylation of the guanine bases in DNA and RNA. A mechanism involving the codon-anticodon structure of the amino acyl transfer RNA and its recognition is proposed.