B. Inouye, Y. Uchinomi, T. Wachi
Mar 1, 1974
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Journal
The Journal of antibiotics
Abstract
In order to clarify the biological effects of rifampicin and its derivatives on livercells, the actions of these antibiotics on the energy transfer reactions were observed in rat liver mitochondria. Rifampicin, 25-desacetyl rifampicin and rifampicin quinone at concentrations of 2.8×10-5-2.7×10-4 M had little effect on the respiration and several energy transfer reactions in rat liver mitochondria, while similar concentrations of 3-formyl rifamycin SV caused a marked decrease in respiratory control and ADP:Oratio with enhancing state 4 respiration, activated Mg++-dependent ATPase and also inhibited ATP-32Pi exchange reaction. These results suggest that 3-formyl rifamycin SV, though not rifampicin itself, acts as a true uncoupler in vitro on rat liver mitochondria and its uncoupling action is fairly powerful.