Li Guang-Lie
2011
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Quality indicators
Journal
Journal of China Medical University
Abstract
Objective To study the effect of temocapril on the level,expression,and activity of elastase in vascular smooth muscle cells (VSMC) in balloon-injured rat artery.Methods The rat model of arterial stenosis induced by artery injury was established.The Wistar rats were treated with temocapril hydrochloride (experimental group) and medicine solution (control group),respectively.On days 2,3,and 5 after the artery injury,the percentages of cells positive for elastase ⅢB and elastase Ⅱ mRNA and the activity of elastase were determined in both groups.Collagenase in situ zymography was performed on day 3 after the artery injury.The intimal area was measured 10 days after the artery injury.Results Compared with the control group,the percentages of cells positive for elastase ⅢB and elastase Ⅱ mRNA,the activity of elastase,the level of type Ⅳ collagenase,and the intimal area significantly decreased in the experimental group.Conclusion Temocapril may inhibit intimal hypertrophy by inhibiting the level,expression,and activity of elastase and the level of collagenase in VSMCs in the injured artery.