S. Yin, Jae-Joon Lee, Yumi Kim
2004
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Quality indicators
Journal
Natural product sciences
Abstract
Previously, hydrochloride has been found to lower dopamine content in PC12 cells (Kim et al., 20001). In this study, the effects of hydrochloride on L-DOPA-induced cytotoxicity in PC12 cells were investigated. Treatment with hydrochloride at concentrations higher than caused cytotoxicity in PC12 cells. In addition, hydrochloride at non-cytotoxic or cytotoxic concentrations significantly enhanced L-DOPA-induced cytotoxicity (L-DOPA concentration, ). Treatment of PC12 cells with hydrochloride and L-DOPA, alone or in combination, also induced cell death via a mechanism which exhibited morphological and biochemical characteristics of apoptosis, including chromatin condensation and membrane blebbing. Exposure of PC12 cells to hydrochloride, L-DOPA and hydrochloride plus L-DOPA for 48 h resulted in a marked increase in the cell loss and percentage of apoptotic cells compared with exposure for 24 h. These data indicate that hydrochloride at higher concentration ranges aggravates L-DOPA-induced neurotoxicity cytotoxicity in PC12 cells. Therefore, it is proposed that the long-term L-DOPA therapeutic patients with hydrochloride could be checked for the adverse symptoms.