R. Dixon, K. Fuller
Nov 1, 1976
Citations
1
Influential Citations
57
Citations
Journal
Physiologial Plant Pathology
Abstract
Abstract Production of the phytoalexin phaseollin occurred in suspension cultures of Phaseolus vulgaris cv. Canadian Wonder without the addition of fungal or chemical inducers. 2,4-Dichlorophenoxyacetic acid at concentrations of 2×10 −5 m and above was strongly inhibitory to cell growth, phaseollin production and phenylalanine ammonia-lyase avtivity, whereas α-naphthylacetic acid up to the same concentrations still permitted phaseollin biosynthesis and growth of the cultures. A positive correlation existed between the extent and timing of increased phenylalanine ammonia-lyase activity and phaseollin production under the different synthetic hormone levels tested. Cell clumps cultured in liquid media produced more phaseollin per unit fresh weigh tha calllus cultures on semi-solid media. It is suggested that physical agitation and altered gas exchange may be responsible for the stimulation of phytoalexin production in suspension cultures.