Yi Zong-chun
2009
Citations
0
Influential Citations
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Citations
Quality indicators
Journal
Journal of Toxicology
Abstract
Objective The impact of benzene metabolite 1,2,4-benzenetriol on proliferation and erythroid differentiation in K562 cells was observed.Methods Cell viability was detected using trypan blue exclusion assay.Propidium iodide staining and flow cytometry analysis were conducted to analyze the cell cycle.Apoptosis was measured by annexin V/propidium iodide staining and flow cytometry.The percentage of cells containing hemoglobin was estimated by staining with benzidine.Results The treatment of 1,2,4-benzenetriol(0.025~0.4 mmol/L) for 24h resulted in a concentration-independent inhibition on cell proliferation in K562 cells.The treatment with 1,2,4-benzenetriol at 0.2 mmol/L for 24h induced an accumulation at S and G2/M phases concomitant with a decrease at G0/G1 phases in K562 cells.The same treatment induced apoptosis in K562 cells with an early apoptotic percentage of 17.93% and a late death percentage of 22.80%.When K562 cells pretreated with 1,2,4-benzenetriol at 0.005 mmol/L for 24 were further treated with hemin to induce erythroid differentiation for 24 h,48 h or 72 h,the hemoglobin-positive percentages in K562 cells at each time point were 21.57%,40.62% and 46.60%,respectively.But the hemoglobin-positive percentage in control K562 cells was 26.23%,48.79% and 53.94%,respectively.Conclusion 1,2,4-benzenetriol inhibited the growth of K562 cell through inducing apoptosis and changing cell cycle,this benzene metabolite also inhibited erythroid differentiation at the non-cytotoxic concentration,which might played an important role in the mechanism of benzene-induced hematotoxicity.