I. Ueda, F. Okumura
Aug 1, 1971
Citations
0
Influential Citations
12
Citations
Quality indicators
Journal
Biochemical pharmacology
Abstract
Abstract The effects of chloroform, diethyl ether and 3-dimethylamino-2-methyl-2-phenoxypropiophenone HC1 (U-0882) were investigated upon crude cyclic 3′,5′-nucleotide phosphodiesterase prepared from dog heart. Chloroform noncompetitively inhibited the enzyme at a partial pressure close to the clinical application. The partial pressure which gave a half-maximal inhibition was estimated to be 30 mb. Diethyl ether, on the other hand, inhibited the enzyme preparation when its partial pressure was above the clinical application and a half-maximal inhibition was induced at 425 mb. The enzyme preparation was inhibited by U-0882 competitively. The concentration which inhibited the enzyme half maximally was about 0.9 mM. A possible role of cyclic AMP in mediating the arrhythmogenic effect of catecholamines is discussed.