He Yu
2002
Citations
0
Influential Citations
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Citations
Quality indicators
Journal
Academic Journal of Guangzhou Medical College
Abstract
Objective:To investigate the neuroprotective effect and mechanism of Cyproheptadine(CPH) on glutamate induced neurotoxicity in rat primary cortical cultures. Methods:The effects of glutamate(Glu) and CPH on neurons morphology were observed by discrepancy microscope. Trypan blue method determined the neuron mortality. The release of Lactate dehydrogenase(LDH) and the content of T SOD were measured in neurons. Results:50 μmol·L -1 Glu obviously increased the mortality and the release of LDH,reduced the activity of T SOD. 10?100 μmol·L -1 CPH could decreased the mortality and the release of LDH,1?10?100 μmol·L -1 elevated the activity of T SOD. Conclusion:CPH has an protective effect on Glu induced neurotoxicity in primary cortical cultures. Its mechanism is related to the increasing of T SOD in neurons,which suggesting the capability of anti lipid peroxide reaction and deleting free radicals enhanced.