Rebekah S. Wood, R. Greenstein, Isabella M Hildebrandt
Nov 5, 2018
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Quality indicators
Journal
Medicinal Chemistry
Abstract
Methylparaben is a commonly used antimicrobial in cosmetics that has been shown to have negative effects on mammalian cells. Human melanoma M624 cells were treated with 1 and 5 mM methylparaben in the presence and absence of 25 mJ/cm 2 ultraviolet B (UV-B) light. Cell proliferation assays showed that 5 mM methylparaben was toxic to M624 cells after 24 hours. Apoptotic signaling pathways were analyzed via isolation of separate cellular compartments and protein analysis via western blot. Upon 5 mM methylparaben treatment, PARP I was cleaved indicating apoptosis, which was medi ated by the TNF-α receptor activated in the lipid rafts of the M624 cells. Upon 25 mJ/cm 2 UV-B radiation, PARP II was activated indicating cellular damage, cytochrome c was released from the mitochondria, and caspase-3 was expressed. Upon combinatory treat ment with 5 mM methylparaben and 25 mJ/cm 2 UV-B, apoptosis was induced through mitochondrial release of cytochrome c, expression of caspase-3 and cleavage of PARP I, while methylparaben-induced TNF-α receptor activation and UV-B-induced PARP II activation was inhibited., demonstrating that antimicrobial methylparaben in cosmetics can cause damage to cells. P, Janssens S, Vandenheede J, Garmyn M, Agostinis P. Activation of p38 MAPK is required for Bax translocation to