Zhang Xiao-bing
2010
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Influential Citations
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Quality indicators
Journal
Chinese Journal of Otology
Abstract
Objective To study injury effects of neomycin sulfate at different concentrations on cultured neonatal rat Cochlear hair cells.Methods The organ of Corti in the whole cochlea from neonatal (P0) SD rats was isolated and cultured for 12 hours.Neomycin sulfate of different final concentrations was added to surviving culture media.Forty samples were randomly selected into a control and 3 test groups (n=10 in each group).Samples in the test groups were treated with neomycin sulfate at 1 mmol /L,2 mmol /L or 4 mmol /L for 24 hours.They were then prepared for confocal microscope examination via immunofluorescence staining of Myosin Ⅶ-a.The number of hair cells within a 100 μm segment was counted on 10 arbitrarily selected images and compared statistically using the CHISS software.Samples in the control group received no neomycin treatment.Results When compared to the control group,the number of inner hair cells in the apical turn showed statistically significant differences in only samples treated with 4 mmol /L neomycin sulfate.However,the number of inner hair cells in the middle and basal turns was statistically different from the control group in all neomycin treated samples.The number of outer hair cells in the apical turn was statistically different from the control group in samples treated with 2 mmol /L and 4 mmol /L neomycin sulfate.The difference in middle and basal turns was of statistical significance for all three neomycin sulfate concentrations.Conclusion The results indicate toxic effects on Cochlear hair cells by neomycin sulfate,with outer hair cells being more sensitive than inner hair cells.The damage appears to start from the basal turn.Increasing neomycin concentration not only increases the severity of hair cell damage,but increasingly involves the middle and finally the apical turn,especially outer hair cells.No hair cell damage was seen in the control group.The ototoxic effects of neomycin sulfate to ochlear cultures are similar to those seen on cochlear hair cells reported in in vivo studies.This can serve as a model for in vitro hair cell regeneration research.