P. B. Barrett, J. Harwood
Jun 1, 1998
Citations
1
Influential Citations
6
Citations
Journal
Phytochemistry
Abstract
Abstract The effects of the thiocarbamate herbicide, pebulate ( S -propylbutylethylthiocarbamate) and its sulphoxide were investigated in vivo . The sulphoxide caused greater inhibition of very long chain fatty acid (VLCFA) synthesis from [1- 14 C]acetate than pebulate, suggesting that the former was the physiologically-active form of the herbicide. In contrast, to fatty acid elongation, de novo synthesis was insensitive to pebulate or its sulphoxide in vivo . The greater sensitivity of elongation to the sulphoxide was confirmed by in vitro assays which measured stearoyl-CoA or arachidoyl-CoA elongation with [2- 14 C]malonyl-CoA. The results confirm suggestions that thiocarbamates are oxidised to their sulphoxide derivatives for full herbicidal activity and it is the latter which inhibit VLCFA and, hence, surface wax synthesis.