H. Morinaka, Y. Kitamura, K. Tsuzuki
1993
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Journal
Journal of Weed Science and Technology
Abstract
Selectivity of pyributicarb [O-3-tert-butylphenyl 6-methoxy-2-pyridyl(methyl)-thiocarbamate] and its effects on lipid biosynthesis were investigated using corn (Zea mays L. cv. Honeybantam) and soybean (Glycine max L. cv. Suzunarihakuchou) as susceptible and tolerant plants, respectively.In water culture, the elongation of young corn roots was more affected by pyributicarb than that of soybean roots.When 14C-acetate was treated with pyributicarb, both uptake into roots and incorporation into the lipid fraction were greater in soybean than in corn. Lipid biosynthesis from acetate seemed to be more active in soybean than in corn roots, and pyributicarb induced the accumulation of squalene in both types of roots. Squalene accumulation in response to the herbicide was also confirmed by gas liquid chromatography without using 14C-acetate, and was obviously larger in corn than in soybean roots.In both root types, pyributicarb decreased the total content of three major unconjugated sterols: campesterol, stigmasterol and β-sitosterol. Reduction of the sterols was considered a result of its inhibition of squalene metabolism. No clear difference was recognized, however, between soybean and corn roots in the amount of sterol reduction.There seemed to be a relation between phytotoxic activity and accumulated amount of squalene caused by the herbicide, suggesting that one action site of pyributicarb was inhibition of the squalene metabolism.